Look at the plasmid map on page 1 of the Exp #7 protocol. If you were to add the restriction enzyme PstI into a tube with this plasmid (and the appropriate buffers) and you were to run the reaction mixture out on an agarose gel, how many bands would you expect to see?
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You have accidentally torn the labels off two tubes, each containing a different plasmid, and now do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids shown in the figure below. You have equipment for agarose gel electrophoresis, a standard set of DNA size markers, and the necessary restriction enzymes. Fill in the following table and determine which restriction enzyme digests would help you identify the samples.
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Lab Report: Isolation of Plasmid DNA from Bacteria 4. If your plasmid is 5,000 bp and a restriction enzyme cuts it once, how many bands would you expect to see on agarose gel electrophoresis? Explain your answer. I would expect to see one band on the agarose gel electrophoresis. Since a 5,000 bp plasmid is cut once, it will result in a single band. 5. If you cloned a 300 bp fragment of DNA into the 5,000 bp plasmid, how many bands and what size bands would you expect to see if you cut this plasmid with the restriction enzyme? Explain WHY? 6. If each of two different restriction enzymes cuts a plasmid once, how many bands would you expect to see on an agarose gel and WHY?
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You have accidentally torn the labels off two tubes, each containing a different plasmid, and now do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in the figure below. You have the opportunity to test just one sample from one of your tubes. You have equipment for agarose gel electrophoresis, a standard set of DNA size markers, and the necessary restriction enzymes. Which restriction enzyme or combination of restriction enzymes would you use in this experiment?
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