Question

3. Make the following dilution series starting with Buffer B as the stock solution. Make 3 1/10 dilutions followed by 1 1/2 dilution. All dilutions should be made in test tubes in a total of 10 mls. Draw your dilution series like the picture the section on serial dilutions. Have your calculations checked before making the dilution series. Buffer 1/10 1 mL -> [10 mL] 9 mL H2O 1/10 1 mL -> [10 mL] 9 mL H2O 1/10 1 mL -> [10 mL] 9 mL H2O 1/2 5 mL -> [10 mL] 5 mL H2O

          3. Make the following dilution series starting with Buffer B as the stock solution. Make 3 1/10 dilutions followed by 1 1/2 dilution. All dilutions should be made in test tubes in a total of 10 mls. Draw your dilution series like the picture the section on serial dilutions. Have your calculations checked before making the dilution series.
Buffer 1/10 1 mL -> [10 mL] 9 mL H2O 1/10 1 mL -> [10 mL] 9 mL H2O 1/10 1 mL -> [10 mL] 9 mL H2O 1/2 5 mL -> [10 mL] 5 mL H2O

3. Make the following dilution series starting with Buffer B as the stock solution. Make 3 1/10 dilutions followed by 1 1/2 dilution. All dilutions should be made in test tubes in a total of 10 mls. Draw your dilution series like the picture the section on serial dilutions. Have your calculations checked before making the dilution series.
Buffer 1/10 1 mL -> [10 mL] 9 mL H2O 1/10 1 mL -> [10 mL] 9 mL H2O 1/10 1 mL -> [10 mL] 9 mL H2O 1/2 5 mL -> [10 mL] 5 mL H2O

Added by Madison O.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Supreeta N

09/19/2023

Step 1

This means you need to take 1 mL of Buffer B and add it to 9 mL of diluent (water or another buffer) in a test tube. This gives you a total of 10 mL of a 1/10 diluted solution. Show more…

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Make the following dilution series starting with Buffer B as the stock solution. Make 3 (1/10) dilutions followed by 1/2 dilution. All dilutions should be made in test tubes, total of 10 mLs. Draw your dilution series like the picture in the section on serial dilutions. Have your calculations checked before making the dilution series.