7. Observe the clinical samples below. Answer the descriptors associated with each micrograph. You may need to observe these online to get the color of the stain. Gram Reaction: Cell Shape: Cell arrangement: Gram Reaction: Cell Shape:
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3. Fill in the chart below. Indicate what color the organism would appear if observed under the microscope after each step (colorless, purple, or pink). Your lecture notes may be helpful in filling out this chart. Reagent Gram + Gram - No reagent Primary stain Mordant Decolorizer Counterstain
Sri K.
Complete the table below for the Gram stain procedure. Include the reagents used in each step and the color that Gram positive and Gram negative cells would appear at that point in the procedure. Reagent Gram Positive Color Gram Negative Color Primary Stain Mordant Decolorizer Secondary Stain
Derrick D.
Read Exercise 3-7 in your lab manual, then complete the following item 3. Why is the order of the reagents in a Gram stain so important? (2 pts) 4. Rarely is a Gram stain ever perfect, so proper interpretation of a Gram stain result requires you to formulate a "consensus" decision about the general Gram reactivity of the cells stained. Study the three microscopic fields of view pictured below and for each develop a consensus decision about the overall Gram reaction of the bacterial cells shown. Record your answers in the blanks provided as either Gram positive, Gram negative, or Gram variable, as appropriate. (3 pts) CELL LEGEND: ● purple ● pink ○ unstained Gram reaction:
Adi S.
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