Question

Name: Bio 230: Microbiology Lab section: Pre-lab handout [1 point] 1. Fill out the table to predict the characteristics of the eight-bacterial species (in bold) mentioned in the reading on pg. 1. Indicate the shape of each bacterium, as coccus, bacillus or spiral. Indicate the color of bacterium after the Gram stain, MSA test and blood test. Use (+) for a positive result and (-) for a negative result in the catalase, oxidase and coagulase tests. Names of "unknown" bacteria Shape Differential stain Gram stain Isolation test MSA test Blood test Biochemical test Catalase test Oxidase test Coagulase test 1 S. epidermis 2 S. aureus 3 S. pyogenes 4 S. pneumoniae 5 E. faecalis 6 B. subtilis 7 P. aeruginosa 2. Based on the table, which test(s) (differential stain, isolation test, or biochemical test) is most helpful in distinguishing between the two Gram-negative rods? 3. An "unknown" bacterium was cultured on nutrient agar. The bacterium turned purple when Gram stained. The unknown presented as individual and clustered rods when observed under the compound microscope at 1000X magnification. Each rod was ~1.0 ?m broad and 2 to 4 ?m long. Biochemical testing showed the bacteria was able to hydrolyze hydrogen peroxide. Based on this summary and your table, what is the unknown bacteria?

          Name:
Bio 230: Microbiology Lab section:
Pre-lab handout [1 point]
1. Fill out the table to predict the characteristics of the eight-bacterial species (in bold) mentioned in the reading on pg. 1.
Indicate the shape of each bacterium, as coccus, bacillus or spiral.
Indicate the color of bacterium after the Gram stain, MSA test and blood test.
Use (+) for a positive result and (-) for a negative result in the catalase, oxidase and coagulase tests.
Names of "unknown" bacteria Shape Differential stain Gram stain Isolation test MSA test Blood test Biochemical test Catalase test Oxidase test Coagulase test
1 S. epidermis
2 S. aureus
3 S. pyogenes
4 S. pneumoniae
5 E. faecalis
6 B. subtilis
7 P. aeruginosa
2. Based on the table, which test(s) (differential stain, isolation test, or biochemical test) is most helpful in distinguishing between the two Gram-negative rods?
3. An "unknown" bacterium was cultured on nutrient agar. The bacterium turned purple when Gram stained. The unknown presented as individual and clustered rods when observed under the compound microscope at 1000X magnification. Each rod was ~1.0 ?m broad and 2 to 4 ?m long. Biochemical testing showed the bacteria was able to hydrolyze hydrogen peroxide. Based on this summary and your table, what is the unknown bacteria?

Name:
Bio 230: Microbiology Lab section:
Pre-lab handout [1 point]
1. Fill out the table to predict the characteristics of the eight-bacterial species (in bold) mentioned in the reading on pg. 1.
Indicate the shape of each bacterium, as coccus, bacillus or spiral.
Indicate the color of bacterium after the Gram stain, MSA test and blood test.
Use (+) for a positive result and (-) for a negative result in the catalase, oxidase and coagulase tests.
Names of "unknown" bacteria Shape Differential stain Gram stain Isolation test MSA test Blood test Biochemical test Catalase test Oxidase test Coagulase test
1 S. epidermis
2 S. aureus
3 S. pyogenes
4 S. pneumoniae
5 E. faecalis
6 B. subtilis
7 P. aeruginosa
2. Based on the table, which test(s) (differential stain, isolation test, or biochemical test) is most helpful in distinguishing between the two Gram-negative rods?
3. An "unknown" bacterium was cultured on nutrient agar. The bacterium turned purple when Gram stained. The unknown presented as individual and clustered rods when observed under the compound microscope at 1000X magnification. Each rod was 1.0 ?m broad and 2 to 4 ?m long. Biochemical testing showed the bacteria was able to hydrolyze hydrogen peroxide. Based on this summary and your table, what is the unknown bacteria?

Added by Martin S.

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