MICROSCOPE LAB Part 1. THINGS TO DO: Determine total magnification of your microscope View the microscope and use the table below to calculate total magnification for each lens (following the example for the 4x): Ocular Lens x Objective Lens = Total Magnification Scanning Objective 10 x 4 = 40x Low Power Objective 10 x High Power Objective 10 x Part 2. THINGS TO DO: Explore Slide Lighting Place any of the provided pre-made slides on microscope stage and answer the following questions: If the slide is not illuminated brightly, what should you do? (list at least two possibilities) If the light is too bright, causing eyestrain, what should you do? (list at least two possibilities) State the inversion phenomenon in your own words:
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Step 1
- Scanning Objective: 10x (ocular lens) x 4x (objective lens) = 40x total magnification - Low Power Objective: 10x (ocular lens) x 10x (objective lens) = 100x total magnification - High Power Objective: 10x (ocular lens) x 40x (objective lens) = 400x total Show more…
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Microscopy Pre-lab Questions These pre-lab questions must be submitted before lab begins; prior to doing any laboratory work. Name: Click here to enter text. 1. Describe the position of your hands while carrying a microscope. Click here to enter text. 2. How do you compute total magnification with different objective lenses of the light microscope? Click here to enter text. 3. What is meant by the term parfocal? Click here to enter text. 4. Why is the 4x objective (scanning objective) placed in position when the microscope is stored or carried? Click here to enter text. 5. What is the relationship between the working distance of an objective lens and its magnification power? Click here to enter text. 6. In what way(s) can you control the amount of light reaching the objective lens? Click here to enter text. 7. List the steps to correctly putting away a microscope: Click here to enter text. 8. What effect does increased magnification have on the field of view? Click here to enter text.
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You have found in the lab an old microscope, which has lost its eyepiece. It still has its objective lens, and markings indicate that its focal length is $7.00 \mathrm{~mm}$. You can put in a new eyepiece, which goes in $20.0 \mathrm{~cm}$ from the objective. You need a magnification of about 200 . Assume you want the comfortable viewing distance for the final image to be $25.0 \mathrm{~cm}$. You find in a drawer eyepieces marked $2.00-, 4.00-,$ and $8.00-\mathrm{cm}$ focal length. Which is your best choice?
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