00:01
Okay, so the question here is that if a de -staining re -age, it was left on for too long when doing a gram stain, what would the conclusion be? and if the de -staining agent was not left on long enough, what would the conclusion be, would you get a false positive or false -negative in both cases? so let's go through the first one.
00:17
De -stating agent was left on for too long.
00:20
So, let's be added in a crystal violet, then we add in the iodine, and then we add in the decolorizer.
00:34
You know what? let's do a different color for iodine.
00:36
Let's do green for iodine.
00:38
Add in the iodine.
00:40
So i only have access to red, black, blue, and green.
00:43
So we'll do green for iodine.
00:46
Alcohol.
00:48
And then this is a decolorizer.
00:49
We add in the decolor racer, and we let it sit for too long.
00:53
And then we add in the saffronin.
00:57
So say too long.
01:01
Okay.
01:02
And then we add in the saffronin.
01:03
So what will the result look like? well, if we have a gram -positive bacteria, it'll be purple, purple all the way through.
01:14
Once it gets decolorized, then it no longer has a color...