Mobile phase: \( 20 \% \) acetonitrile, \( 80 \% \) water - isocratic
Temperature: \( \mathbf{4 0} \mathrm{C} \)
Flow rate: \( 1 \mathrm{ml}_{\mathrm{min}^{-1}} \)
Detector: UV-visible spectrophotometer.
Figure 2 Chromatogram to show an HPLC analysis of a mixture of aminoacids, including phenylalanine and tyrosine (peaks 1 and 2 ).
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\( 15^{\circ} \mathrm{C} \) Rain showe: