Multiple Choice Question A change in the nucleotide sequence coding for a protein is called a O ligation. O mutation. O promoter. O transformation. Need help? Review these concept resources. Rate your confidence to submit your answer. High Medium Low
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Usually, a mutation in the promoter region of an operon causes reduced levels of synthesis of the proteins encoded by that operon. Occasionally, a mutation in the promoter region actually causes increased levels of synthesis. Can you suggest (briefly) a plausible explanation? 2. A DNA sequence that may be present as only a single copy in a large mammalian genome can be amplified and cloned using the polymerase chain reaction (PCR). Describe the steps and reaction components required in a PCR experiment. Illustrate the steps in just one round. 3. Suppose a biochemist has just developed a technique for the efficient replacement of mutant genes with normal ones in human germ line cells. She proposes to use this technology to eliminate familial hypercholesterolemia from the human gene pool. Similar projects are being proposed in several other countries. Should the U.S. National Institutes of Health allow her to proceed? Why or why not? 4. What is a DNA microarray? How does it resemble and how does it differ from a DNA library? 5. Which would you expect to be larger, the percentage of the human genome that is translated into protein, or the percentage of the genome of a bacterium that is translated into protein? Why?
Adi S.
Any change in the nucleotide sequence of DNA is called an anticodon. a base substitution. a mutation. a mutagen.
A.) A eukaryotic DNA sequence that affects transcription at distant promoters is called a(n) _______. a) Regulatory promoter b) Enhancer c) Core promoter d) Activator e) Mediator B.) The yoda regulator protein binds to regulator sequences and stimulates transcription of the jedi operon. When the yoda protein binds to a small molecule, it is no longer capable of binding to DNA near jedi. The jedi operon is always expressed ("always on") if a mutation prevents the yoda protein from binding to the small molecule. Control of this operon is: a) 1. Positive inducible b) 2. Positive repressible c) 3. Negative inducible d) 4. Negative repressible C.) Which of the following is an example of a spontaneous mutation? a) The base analog, 5-bromouracil, causes a mutation from adenine to guanine. b) The intercalating agent, acridine orange, causes the insertion of a guanine. c) Ultraviolet radiation causes covalent bonds between a cytosine and an adjacent thymine on the same strand, that are then replaced with two thymines. d) Wobble causes a guanine to be substituted for an adenine. D.) A gene with the mRNA sequence: 5'-UAC UGG CCG UUA UGC GUU GAU AUA ACG-3' and produces a peptide with the amino acid sequence: Tyr Trp Pro Leu Cys Val Asp Ile Thr. A mutation in this gene creates a protein with the following sequence: Tyr Trp Pro Leu. What type of mutation is this? Select the best answer based on the information given. a) 1. Nonsense mutation b) 2. Missense mutation c) 3. Silent mutation d) 4. Suppressor mutation e) 5. Gain of function mutation f) 6. Reverse mutation E.) A gene with the mRNA sequence: 5'-UAC UGG CCG UUA UGC GUU GAU AUA ACG-3' and produces a peptide with the amino acid sequence: Tyr Trp Pro Leu Cys Val Asp Ile Thr. A mutation in this gene creates a protein with the following sequence: Tyr Trp Pro Leu Tyr Val Asp Ile Thr. What type of mutation is this? Select the best answer. a) 1. Deletion b) 2. Silent mutation c) 3. Somatic mutation d) 4. Base substitution e) 5. Insertion
Shaiju T.
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