Observations and Results PART A: Isolation of Discrete Colonies from a Mixed Culture STREAK-PLATE TECHNIQUE S. marcescens and M. luteus M. luteus and E. coli Draw the colonies that appear on each agar plate. Colony description: Form Elevation Isolate 1 Isolate 2 Isolate 3 Isolate 4 Pigmentation RED yellow Yellow cream Size circle small small medium S. marcescens M. luteus M. luteus E. Coli
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Observations and Results PART A: Isolation of Discrete Colonies from a Mixed Culture STREAK-PLATE TECHNIQUE
Adi S.
Identify the isolated colonies: Pseudomonas aeruginosa, Salmonella sp., Staphylococcus epidermidis, Escherichia coli, Shigella sp. Colony 1: Colony 2: Colony 3: Media MSA agar. EMB agar. MCK agar. SS agar. Blood agar. Colony 1 No growth Irregular colorless colonies Irregular colorless colonies Irregular colorless colonies Large colonies with beta hemolysis Colony 2 No growth Colorless colony Colorless colony Colorless colony White colonies, with gamma hemolysis Colony 3 No growth Green metallic colonies Purple colonies with precipitated bile salts Pinkish colonies White colonies gamma hemolysis Identify the isolated colonies:
RESULTS: Techniques for Isolation of Pure Cultures Streak plate Small yellow = Micrococcus luteus Red = Serratia marcescens Cream = Escherichia coli Agar slant 1 Agar slant 2 Agar slant 3 M. luteus S. marcescens E. coli
Sri K.
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