PART 3: Translating mRNA to protein After RNA processing, the mature mRNA is exported from the nucleus to the cytoplasm. At this point the mature mRNA can be translated based on its reading frame and the genetic code. The position of the start codon defines the reading frame of the mature mRNA. Identify and underline the start codon in the mRNA sequence that you wrote out in Box II. *Hint: In the gene Q mRNA the start codon is located closest to the 5’ end of the mature mRNA. Once the reading frame start is located, the rest of the codons can be identified by organizing the nucleotides into contiguous sets of three at a time. The end of the reading frame is defined by the presence of one of the three possible stop codons in the same frame.
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Step 1: The start codon in the mRNA sequence provided in Box II is AUG. Show more…
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1. The entire (very small) mRNA sequence encoded by the human XAM gene is given below. The stop codon is capitalized. 5'-augucccauccuuuucaccaggaugggagagguugcuucccucccacauccggcaugcgcauuaUAAaagaugauaauauaaag-3' a. (1 point) When the mRNA above was transcribed, what were the first 5 nucleotides of the DNA template strand which were "read" by RNA polymerase? Choose one. a. 5'-ATGTC-3' b. 3'-TACAG-5' c. 5'-ATAAAG-3' d. 3'-TATTTC-5' e. 5'-ATGGG-3' f. 3'-TACCC-5' b. (1 point) The gene encoding this mRNA contains one intron. Which of the following correctly describes the intron's relationship to the mature mRNA? a. The intron is not in the mature mRNA because it was removed during splicing. b. The intron is not in the mature mRNA because it was not transcribed. c. The intron is in the mature mRNA, at its 3' end. d. The intron is in the mature mRNA, at its 5' end.
Sri K.
$\begin{array}{|c|c|}\hline \text { Codon on mRNA } & {\text { Amino Acid }} \\ \hline \mathbf{G C A} & {\text { alanine }} \\ \hline \mathbf{A A G} & {\text { lysine }} \\ \hline \mathbf{G U U} & {\text { valine }} \\ \text { AAU } & {\text { asparagine }} \\ \hline \mathbf{U G C} & {\text { cysteine }} \\ \hline \mathbf{U C G} & {\text { serine }} \\ \hline \mathbf{U C U} & {\text { serine }} \\ \hline \text { UUA } & {\text { leucine }} \\ \hline \text { UAA } & {\text { stop }} \\ \hline\end{array}$ You are given three mRNA sequences: 1. 5’-UCG-GCA- AAU-UUA -GUU-3’ 2. 5’-UCU-GCA- AAU-UUA -GUU-3’ 3. 5’-UCU-GCA- AAU-UAA -GUU-3’ Using the table, write the peptide encoded by each of the mRNA sequences. a. 1. Serine-alanine-asparagine-leucine-valine 2. Serine-alanine-asparagine-leucine-valine 3. Serine-alanine-asparagine(-stop) b. 1. Serine-phenylalanine-asparagine-leucinevaline 2. Serine-alanine-asparagine-leucine-valine 3. Serine-alanine-asparagine (-stop) c. 1. Serine-alanine-asparagine-leucine-valine 2. Serine-alanine-asparagine (-stop) 3. Serine-alanine-asparagine-leucine-valine d. 1. Serine-alanine-asparagine-leucine-valine 2. Serine-arginine-asparagine-leucine-valine 3. Serine-alanine-asparagine(-stop)
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