Question

Part II: Interpreting Data & Drawing Conclusions In the growth curve below, Pseudomonas aeruginosa was grown the OD600 measure hourly over 12 hours. Use the data in the graph and the calculations above to answer the questions below. Notice that the numbers above the data point are the OD600 for that timepoint. Growth of Pseudomonas aeruginosa 10 1 OD600 0.1 0.01 0.078 0.315 0.999 2.29 3.155 3.915 3.465 3.895 4.595 4.905 4.9 5.275 0 2 4 6 8 10 12 14 Hours 1) At timepoint 8 hours, calculate the population density in CFU/ml (Show your work) 2) Given the data, determine the generation time for this organism during log phase 3) At what time (approximately) does this organism enter Stationary phase? 4) This growth curve was conducted at 37°C, what do you think would happen if the growth curve was conducted at 25°C?

          Part II: Interpreting Data & Drawing Conclusions
In the growth curve below, Pseudomonas aeruginosa was grown the OD600 measure hourly over 12 hours.
Use the data in the graph and the calculations above to answer the questions below. Notice that the numbers above the data point are the OD600 for that timepoint.
Growth of Pseudomonas aeruginosa
10
1
OD600
0.1
0.01
0.078
0.315
0.999
2.29
3.155
3.915
3.465
3.895
4.595
4.905
4.9
5.275
0
2
4
6
8
10
12
14
Hours
1) At timepoint 8 hours, calculate the population density in CFU/ml (Show your work)
2) Given the data, determine the generation time for this organism during log phase
3) At what time (approximately) does this organism enter Stationary phase?
4) This growth curve was conducted at 37°C, what do you think would happen if the growth curve was conducted at 25°C?

Part II: Interpreting Data Drawing Conclusions
In the growth curve below, Pseudomonas aeruginosa was grown the OD600 measure hourly over 12 hours.
Use the data in the graph and the calculations above to answer the questions below. Notice that the numbers above the data point are the OD600 for that timepoint.
Growth of Pseudomonas aeruginosa
10
1
OD600
0.1
0.01
0.078
0.315
0.999
2.29
3.155
3.915
3.465
3.895
4.595
4.905
4.9
5.275
0
2
4
6
8
10
12
14
Hours
1) At timepoint 8 hours, calculate the population density in CFU/ml (Show your work)
2) Given the data, determine the generation time for this organism during log phase
3) At what time (approximately) does this organism enter Stationary phase?
4) This growth curve was conducted at 37°C, what do you think would happen if the growth curve was conducted at 25°C?

Added by Jose Miguel H.

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