Pictured below is the DNA recognition and cut site for the restriction enzyme BamHI. What type of cut does BamHI produce? Question 8 options: A cut with 5' overhangs None of these choices A cut with 3' overhangs A cut with blunt ends
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Shaiju T.
The palindromic recognition sequence for the restriction enzyme BamH1 is $5^{\prime}$ GGATCC $3^{\prime} .$ Remember, the other strand of DNA will have the complementary set of bases and will read the same as this sequence, but just "backwards." The enzyme cuts between the first and second Gs in the sequence. What is the "sticky-end" sequence when the enzyme cuts double-stranded DNA? a. $5^{\prime}$ GGATTC $3^{\prime}$ c. $5^{\prime}$ GATCC $3^{\prime}$ b. $5^{\prime}$ CCATGG $3^{\prime}$ d. $5^{\prime}$ CCATG $3^{\prime}$
Biotechnology
Enzymes Allow the Creation of Recombinant Molecules In Vitro
Consider the following restriction enzyme sites: EcoRI KpnI BamHI SalI HindIII 5'GAATTC 3'CTTAAG 5'GGTACC 3'CCATGG 5'GGATCC 3'CCTAGG 5'GTCGAC 3'CAGCTG 5'AAGCTT 3'TTCGAA Which of the restriction enzymes generates 3' overhangs? Select one: a. EcoRI; BamHI; SalI, HindIII b. All of them because for every 5' overhang there must be a corresponding 3' overhang c. None of them d. KpnI
Sri K.
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