Plasmid vectors often contain ______ genes that can be used to screen for recombinants insertion sequence Oselective marker Opromoter/operator metabolic activation
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Step 1: Plasmid vectors are small, circular DNA molecules that are used as vehicles to carry foreign DNA into cells. Show more…
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A plasmid suitable for use as a cloning vector must contain which two features? a. a gene for positive selection, such as an antibiotic resistance gene, and a sequence that recognizes an activator b. a restriction enzyme cleavage site and a gene for positive selection, such as an antibiotic resistance gene c. a gene for a restriction endonuclease and a gene for positive selection, such as an antibiotic resistance gene. d. an operator and a promoter e. a restriction enzyme cleavage site and an operator and a gene for a repressor protein
Shveta P.
Selecting for Recombinant Plasmids When cloning a foreign DNA fragment into a plasmid, it is often useful to insert the fragment at a site that interrupts a selectable marker (such as the tetracycline-resistance gene of $\mathrm{pBR} 322$ ). The loss of function of the interrupted gene can be used to identify clones containing recombinant plasmids with foreign DNA. With a yeast artificial chromosome (YAC) vector, it is not necessary to do this; the researcher can still distinguish vectors that incorporate large foreign DNA fragments from those that do not. How are these recombinant vectors identified?
The cloning vectors in use today contain an origin of replication, a selectable marker gene (usually an antibioticresistance gene), and one additional component. What is this component, and what is its function?
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