Q.4 Our researcher's colleague said all of this was a waste of time and that all that was required was to transfect the 'fluffy' transcription factor into some cells and then use RT-qPCR to see if recombinant expression of 'fluffy' results in changes in the expression of 'fido' mRNA. What are the potential limitations of this approach with respect to proving that 'fluffy' is a transcription factor?
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Step 1: Restate the colleague's proposal concisely: transfect cells with recombinant "fluffy" and use RT‑qPCR to see whether "fido" mRNA levels change, then conclude "fluffy" is a transcription factor. Show more…
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In an experimental situation, a student researcher inserts an mRNA molecule into a eukaryotic cell after she has removed its 5' cap and poly-A tail. Which of the following would you expect her to find?: The mRNA is directed only to ribosomes bound on the rough ER when the 5' cap and 3' poly A tail are missing The cell quickly adds a new poly-A tail to the mRNA The molecule is degraded rapidly because it is not protected at the 5' and 3' end The mRNA has increased translation rates since the 5' cap and 3' poly-A tail usually slows translation The mRNA is quickly converted to a ribosomal subunit
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