Q7. Why did you streak the competent cells on your plates before attempting transformation? Q8. Why did you streak the cells that you transformed onto the "amp -" plate? What does it mean if they didn't grow? Q9. How did the forms of growth of transformed cells compare between the "amp -" plate and the "amp +" plate? Why did you observe this difference? Q10. Name two methods you could use to check and see if our bacterium had the ampicillin resistance gene. Q11. Explain how a transformed bacterium may acquire and then display a new phenotype, e.g., antibiotic resistance or fluorescing cells.
Added by Jeanne A.
Step 1
Streaking the competent cells on plates before attempting transformation is done to ensure that each colony on the plate arises from a single transformed cell. This allows for the isolation and identification of individual transformed colonies. Q8. The cells that Show more…
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