QUESTION 1 Which type of radiation uses shorter wavelengths? ionizing nonionizing QUESTION 2 Ionizing Radiation Damages DNA by forming thymine dimers producing free radicals burning none of these QUESTION 3 Non-ionizing Radiation damages DNA by forming free radicals burning forming thymine dimers none of these QUESTION 4 Non-ionizing radiation can penetrate paper skin concrete none of these because it doesn't penetrate very well at all QUESTION 5 What is the Genus and species of the bacteria that you will be using in this experiment? E. coli Serratia marcescens Bacillus cereus Salmonella enterica QUESTION 6 How many plates will you prepare? 1 2 3 4 QUESTION 7 Plate #3 will be covered with the lid and exposed to UV light for 3 minutes be covered with the lid and exposed to UV light for 10 seconds be uncovered and exposed to UV light for 10 seconds be uncovered and exposed to UV light for 3 minutes QUESTION 8 Cardboard cutout masks will be used on plate 1 plate 2 plate 3 all of the plates QUESTION 9 The plates will be incubated at 37 degrees Celsius 25 degrees Celsius 50 degrees Celsius 25 degrees Fahrenheit QUESTION 10 The name of the pigment that Serratia produces at 25 degrees Celsius is called pyocyanin methylene blue prodigiosin none of these
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Which type of radiation uses shorter wavelengths? Answer: ionizing Show more…
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E. Effect of UV Radiation Record the results for each plate at follows: 0 no growth + light growth ++ medium growth +++ heavy growth Also note any differences in appearance of growth on each of the plates. Bacteria | Visible light | UV lid on | UV lid off 5 sec | UV lid off 10 sec | UV lid off 1 min | UV lid off 10 min Serratia marcescens | ++ | +++ | +++ | +++ | ++ | +++ Bacillus subtilis | + | +++ | +++ | +++ | ++ | 0 Based on these results, answer the following questions: 1. Did visible light have any effect on microbial growth? 2. Did UV exposure with the lid on have any effect on microbial growth? 3. Explain any differences you observe between the two species tested. 4. Describe any other differences in the appearance of colonies on the irradiated plates.
Madhur L.
Procedure pGLO Bacterial Plates - liquid medium (liquid culture) and solid medium (plate culture): For this experiment, you will be adding nothing (control) to one plate and arabinose to the other plate. After letting the plates sit at room temperature for 10 minutes, you will add bacteria to the plate using an inoculating loop and incubate the plates overnight at 37°C. Students will then examine each plate under UV light to determine if any of the sugars were able to activate transcription of the GFP gene. Protocol Add 100 αl of bacteria (competent cells produced from previous lab) to each of the four Eppendorf tubes. To tubes 1 and 2, add 10 αl of H2O. To tubes 3 and 4, add 10 αl of pGLO DNA. Be sure that the DNA gets into the tube. Incubate all samples on ice for 15 minutes. Place the samples at 42°C for 90 seconds. Place the samples on ice for 2 minutes. Add the contents of tubes 1-4 to plates 1-4 (plate 1: LB/amp, plate 2: LB/amp, plate 3: 5% D-arabinose, plate 4: LB/amp + arabinose). Incubate the plates overnight at 37°C. Determine which plates had bacterial growth, which had individual colonies, and which were empty. Questions: What type of growth would you expect on each plate? Why? Do some of the bacterial plates glow green and others don't? Why? If you had bacteria that did not glow, could you do something to make them glow? If so, how?
Prolonged exposure to UV radiation can be lethal to cells because UV radiation alters the DNA and RNA by forming covalent bonds where they don't belong and disrupting base pairing during DNA replication and transcription. Why does disrupting base pairing during DNA replication and transcription kill cells? There has been talk recently about using UV radiation as a way to kill SARS-CoV-2 (the virus that causes COVID-19) on surfaces like N95 masks, cardboard, plastic, and other inanimate objects. Several studies say that it works. Based on what you know about UV radiation and viruses, does it make sense that UV radiation works for this purpose? Explain your answer. There was even a suggestion that UV radiation should be used as a treatment for patients with COVID-19... we could just shine big, intense UV lights at them (or in them?), and it will kill the virus and save the patient. What do you think? Should we use intense UV radiation to treat people with COVID-19? Why or why not? Identify the variables for the experiment described above. Independent variable: Dependent variable: Control variables:
Dominador T.
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