Question 11 (2 points) Size of DNA Fragment vs. Distance...

Question 11 (2 points) Size of DNA Fragment vs. Distance migrated 25000 20000 15000 10000 5000 0 0 5 10 15 20 25 30 35 40 45 Size fragment (bp) y = 42929e^-0.12x R² = 0.8264 Distance migrated (mm) According to the graph, how large is a PCR fragment that migrated 30 millimeters? Explain how you calculated its size.

Consider two fragments of DNA of radius $r_{1}=30 \mathrm{nm}$ and $r_{2}=35 \mathrm{nm}$ when they are coiled up as they move through a gel. An electrophoresis analysis (Fig. 17.38 ) is carried out to separate these two fragments. Suppose the drag factor $C$ in Equation 17.19 is $C=5.0 \times 10^{-5} \mathrm{kg} /(\mathrm{m} \cdot \mathrm{s})$, and assume each fragment has a charge $q=-10 e$ a. If the electric field has a magnitude of $1.0 \mathrm{N} / \mathrm{C},$ what is the speed of each fragment in the gel? b. How long will it take the smaller fragment to move a distance $1 \mathrm{cm}$ (a typical distance used in DNA fingerprinting)? c. After the time in part (b) has elapsed, what is the separation of the two different-size fragments in the electrophoresis tube?

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