Question 1 (1 point) If one strand of a chromosome includes the sequence 5'...CTTGTCAG...3', the sequence of the other, complementary strand from 5' to 3'is 5'...\ldots3. Question 2 (2 points) Among the four bases present in DNA, A and \ldots are classified as purines, and T and \ldots are classified as \ldots Question 3 (1.5 points) Consider a double helix of DNA that has a total of 50 base pairs. If 32 of the bases are A (adenine), then \ldots of the bases should be C, \ldots should be G, and \ldots should be T. (Be sure to enter the number of each base, rather than the percentage.) Question 4 (1 point) Calculating the base compositions in the previous problem involves the application of rules that were first discovered by Erwin \ldots Question 5 (1 point) Which of the following is/was a feature of the Pauling-Corey ("P-C") model for DNA (i.e., the structure proposed by Linus Pauling and Robert Corey, as opposed to that of Watson and Crick)? a) The bases in the P-C model are located on the inside of the helix. b) The helix in the P-C model consists of 3 DNA strands. c) The phosphates in the P-C model are negatively-charged. d) The P-C model assumed that both T and U are present in DNA. e) All of the above
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In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaff's rule, reconstruct the missing data and list the base composition of the DNA sample. (Include a brief explanation of how you got your results).
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Chapter 9 Review: Molecular Genetics Learning Objectives and Application Questions Learning objectives are identified for you to review your understanding of this topic. You are advised to reflect carefully on these objectives and check your own understanding to see if you have understood these essential concepts from this week's reading. Answer thoroughly all the application questions associated with each objective in your own words. Learning Objective: Describe the structure of DNA (p. 202, 203). Application question #1: Match the following scientists with their corresponding discovery. Note: one item on the right will be used more than once. - Rosalind Franklin - Erwin Chargaff - James Watson - Francis Crick - Maurice Wilkins A. Credited with discovering the Double Helix model of DNA B. Provided the basis for pairing complimentary DNA bases C. Used X-ray crystallography to discover DNA's helical shape Application question #2: Write in the missing words in the statement below. The building blocks of a DNA molecule are called _______. Each one of these building blocks consists of the 5-carbon sugar _______, a _______, and one _______ base. There are four bases in DNA called _______, _______, _______, and _______. Application Question #3: There is a strict rule specifying how DNA bases in the double helix model should pair. This rule states that the base Adenine must always pair with _______, and _______ must always pair with _______. Learning Objective: Explain the semi-conservative process of DNA replication and repair in eukaryotes (p. 206-207). Application Question #4: In your own words (do not copy-paste an answer from any source), describe the meaning of semi-conservative replication. Application Question #5: Use the pairing rules of semi-conservative replication to copy (replicate) the single strand of DNA provided below: DNA Template: ATTCGATCGACG DNA Replica:
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For the DNA sequence 3'-G-C-C-T-A-T-5' in one DNA strand, the sequence found in the complementary DNA strand must be: A. 5'-C-G-G-A-T-A-3'. B. 5'-C-G-G-A-T-A-3'. C. 3'-G-C-C-T-A-T-5'. D. 5'-G-C-C-T-A-T-3'. 2. Leading and lagging DNA strands exist at a replication fork because ___________. A. the two strands of DNA are antiparallel, and DNA polymerase can only add new nucleotides onto a 3' end. B. the two strands of DNA are parallel and DNA polymerase can only add new nucleotides onto a 3' end. C. the two strands of DNA can be either parallel or antiparallel. D. the two strands of DNA are antiparallel, and DNA polymerase can only add new nucleotides onto a 5' end. 3. The enzyme responsible for removing the RNA primer during prokaryotic DNA replication is _________. A. DNA polymerase alpha. B. RNAse H. C. DNA polymerase I. 4. The enzyme Telomerase __________. A. seals the gaps between Okazaki fragments. B. removes RNA primer and replaces it with newly synthesized DNA. C. adds DNA to the end of linear chromosome.
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