00:01
Hello and we are going to demystify around six questions here.
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We are going to discuss what answers best should them.
00:11
The first question is focusing on, you know, recombinant dna technology is a very important, it's a very important topic especially in contemporary research and technology, research and development.
00:26
There is a question talking about recomminant, recombin dna technology and recombinant is a phenomenon where we combine genes from organism a and we combine genes from organism b to make a recombinant molecule ab or a chimera that bears both of these suitable genes and most often oftentimes this ab recombinant organism here has superior traits than either of these parents okay or if it's a plant, it has qualities maybe to resist pests or to resist insecticides, some sort of in the direction.
01:17
So when we are combining molecules from different organisms, step one is to cut.
01:24
Step one is to cut the genes from either of these organisms and we use substances or molecules we call molecular scissors.
01:36
Molecular scissors.
01:39
Let me just call the molecular scissors because they are actually what they used to cut or excise.
01:44
Excise is a correct term to use here excise.
01:47
And most of the times these are enzymes called endoneuclesis, endo -neclases, endo because they act in the gene within the nucleus and the nucleus they cleave that particular gene.
02:01
So this is the result of this cut is a double -stranded that break specifically where the cut is needed.
02:12
After afterwards we have what we call molecular glues.
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Molecular glues are what we call molecular glouzer or let me just call them enzyme i guess.
02:27
These are the ones that now seal these cuts and they seal the cuts and they're like the hill the the cut site okay so make sure that these trojans combine effectively and they incorporated or let me say they're integrated into this genome of these superior species so among the answers if you check in you see endoconucleus and legase those should be the correct answer and that should be option d endonuclis and legase enzyme let's move on to b question b other what's a plasmid now let's talk about plasmid plasmids important molecules when we're dealing with recombinant dna technology, okay? and plasmids are like vehicles.
03:23
If you cut a gene from organism a, this is a gene, cut the codes for maybe base resistance or antibiotic resistance, and you want to insert it in organism x.
03:38
You're going to use molecular vehicle, or we call it molecular vehicle because you're going to insert this particular gene in a plasmid and a circular extrachronosomal molecule that can cooperate effectively this gene of interest and you need to understand also that we extract these placements from bacteria and an example of a good plasma is plasmid university of california as a common list to carry up these recommend technologies processes so you you get your plasmid you excise and you need to excise or you cut it using the same restriction enzyme used to cut your gene of interest here so you can use ikori you can use bacilli amyloquifurions i can also use there are the number of assyrian as a mechanism can use here.
04:37
So if you cut your gene of interest using ikori, you ensure that you cut your plasmid using the same enzyme so that sticky ends are created.
04:48
Sticky ends will ensure that that gene is well incorporated, okay? sticky ends and i'll show you later what sticky ends mean.
04:58
So this means that the plasmid here serves as a vehicle.
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So you'll incorporate your gene here and then insert it inside the bacteria.
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And then incorporate this bacteria into the system you want.
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And that will mean that your gene will be effectively transferred.
05:17
So the choice answer for b should be a, rather.
05:23
This is question four...