00:01
In this video today we're looking at restriction enzymes.
00:11
And restriction enzymes are special classes of enzymes used to cut dna sequences.
00:19
You can say cut, dna, to make specialized cuts are dna sequences called palindromic sequences.
00:29
Okay.
00:31
And this palindromic sequences are sequences that can be read on either side, the same, okay? the same on either sides, opposite sides.
00:46
I mean, for instance, if you have the particular sequence in reading 5 -3, that sequence can be the same way in the 3 -5 direction, opposite direction.
00:58
And these enzymes can cut these sequences to enable for processes like gene cloning and all those analysis, all right? but then they have a style, you know, they have a specific way of cutting the sequences.
01:11
They can either cut in the middle, and in the middle, if the enzymes cut in the middle of the sequences, they produce a blunt, because this is just a sequence you cut the middle, it produce a blunt one.
01:29
But then there is also they cut in a staggering fashion.
01:36
Okay so if they cut this sequence in a staggering fashion they produce sequences that are sticky and sticky and good for gene cloning for instance because they ensure compatibility between cloning vector and host all right but then we are given we've been given a list of sequences here so any sequence that is palindromic therefore qualifies for that so we have the first sequence is 5 prime you have gaa ttc 3 prime so can we do a complementary sequence so this is 3 prime this is c t t t a a g this is 5 prime so as you can see this sequence if you read it 5 3 direction and 3 5 5 direction is the same thing g a t tc for the upper sequence and g a t tc for the lower sequence so this automatically is a palindrome sequence.
02:45
So that enzyme can cut either in the middle or can cut in a staggering fashion targeting between adenine and guanine.
02:56
So this for instance can be a cut and this cut will be staggering, okay, which is producing sticky ends.
03:04
For sequence number two we have five prime.
03:08
We have g g g there are four gs there and also we have have four cs, one, two, three, four, this is three prime.
03:20
So if we do a complementary sequence to these, therefore we'll have three prime, c c, c, and g, and five prime.
03:31
Also, this is a palindromic sequence.
03:34
So that enzyme and also decide to cut either in that fashion, the targeting between g...