Question 8 Imagine you are asked to extract some DNA from skin cells from someone. Which of the following would be used to amplify a specific section of DNA from that extraction? Polymerase Chain Reaction Genetic Modification Recombinant DNA Technology Restriction Enzymes Question 9 Imagine you are taken captive by a bunch of Biology majors who are stressed about final exams. They have to make a bacteria that has an antibiotic resistance gene inserted into its DNA. Which of the following would you use to help out your captors? Molecular Cloning Polymerase Chain Reaction Chromosomal Duplication Electrophoresis
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Use a method such as swabbing or scraping to obtain the cells. Then, proceed to extract DNA from these cells using a chemical extraction method, which typically involves breaking open the cells (lysis), removing proteins and other cellular components, and then Show more…
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Lesson 1 DNA Template Preparation Focus Questions 1. Why is it necessary to chelate the metal ions from solution during the boiling/lysis step at 100°C? What would happen if you did not use a chelating agent such as the InstaGene matrix? 2. What is needed from the cells for PCR? 3. What structures must be broken to release the DNA from a cell? 4. Why do you think the DNA is stored cold with the InstaGene matrix after boiling the samples?
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Rank the following steps in the correct order that you would use to isolate DNA from strawberries. Rank the options below: 1. wait for DNA precipitate to form 2. transfer strawberry filtrate to test tube 3. spool DNA precipitate onto glass rod 4. add ice cold ethanol to test tube 5. dissolve meat tenderizer and add it to test tube
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