Which of the following is the correct sequence for the aseptic transfer of a bacterial culture? 1. Remove the lid of the media receiving the culture & flame the mouth of this tube. 2. Flame the mouth of the culture tube. 3. Insert the wire loop with the culture to inoculate the bacteria on or in the new media. 4. Reheat the mouth of the culture tube and replace the cap. 5. Sterilize the loop by heating it from base to tip uniformly orange-hot. 6. Get a loopful of the culture. 7. Remove the cap from the culture tube and hold it with your pinky finger of your dominant hand.
Added by Daniel D.
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First, we need to sterilize the loop: **5** Show more…
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Sri K.
Text: Select all of the TRUE statements regarding proper aseptic culturing technique? (Mark all that apply) a. When inoculating new media, all tubes and plates should be clearly labeled before any transfers are made. b. When flaming a loop, begin at the base, then slowly pull the loop through the flame until it is red hot. c. Blow gently on the sterilized inoculating loop to make sure it is cool enough to pick up the culture. d. After removing the cap of the culture tube, keep it in your hand pointed down to minimize the risk of contamination. e. When subculturing from a broth, the tube should be gently mixed before transfer.
Order the steps correctly for transferring culture from an agar slant to a sterile slant. Remove loop - flame tube opening - replace cap Remove cap with little finger do not put cap down Touch loop to top of medium where there is growth and pick up a small amount Pick up sterile slant - open cap with one hand - insert loop Sterile loop Label sterile slant with name - date - medium - organism Flame the loop Move loop in a zig zag motion from bottom to top of medium in slant Flame the tubes opening and insert loop Withdraw loop - flame tube top - replace cap
Shaiju T.
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