QUESTION 6 Mike, as student in BIO 225 was performing a catalase test and set up a positive control with a known Catalase Positive bacteria obtaining the expected bubbles when the hydrogen peroxide was added to the bacteria sample. However, before he could perform the test with his unknown test bacteria sample he spilled the remaining Hydrogen Peroxide from his lab kit supplies so he used some that he had in his home medicine cabinet. No bubbles were produced after the addition of the hydrogen peroxide. What explanation of these results would you give Mike? Mike should conduct a test with a known Catalase Positive bacteria using the hydrogen peroxide from his medicine cabinet before interpreting his results Mike's test bacteria sample is catalase negative Mike's test bacteria sample is catalase positive Mike can only use eScience supplied hydrogen peroxide for this catalase test QUESTION 7 The term "sticky ends" refers to the sticky/gooey DNA obtained in extraction procedures. True False
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Mike, as a student in BIO 225, was performing a catalase test and set up a positive control with a known Catalase Positive bacteria obtaining the expected bubbles when the hydrogen peroxide was added to the bacteria sample. However, before he could perform the test with his unknown test bacteria sample he spilled the remaining Hydrogen Peroxide from his lab kit supplies so he used some that he had in his home medicine cabinet. No bubbles were produced after the addition of the hydrogen peroxide. What explanation of these results would you give Mike? Mike should conduct a test with a known Catalase Positive bacteria using the hydrogen peroxide from his medicine cabinet before interpreting his results. Mike's test bacteria sample is catalase negative. Mike's test bacteria sample is catalase positive. Mike can only use eScience supplied hydrogen peroxide for this catalase test. The term "sticky ends" refers to the sticky/gooey DNA obtained in extraction procedures. True False
Adi S.
B. Suppose you performed oxidase and catalase tests on the following organisms and obtained these results: Organism | Appearance after adding OxiDrop | Appearance after adding H2O2 E. coli | No change | bubbles Pseudomonas aeruginosa | purple | bubbles Enterococcus faecalis | No change | No change Bacillus cereus | No change | bubbles 1. Which organism(s) were oxidase-positive? 2. Which organism(s) were catalase-positive? 3. Which organism(s) has cytochrome oxidase? 4. Can an organism be oxidase-negative and still carry out aerobic respiration? Explain. 5. Can an organism be catalase-negative and still be able to survive in the presence of oxygen? Explain. 6. Based on all of the results of all these tests, one of these organisms does not carry out respiration. Which one? How do you know? 7. Suppose you performed the oxidase and catalase tests on a strict anaerobe. What results would you expect (positive or negative)? Be sure to include both tests.
Sri K.
1) Based on the virtual lab "catalase test", which bacteria was positive (+) or negative (-)? bacteria | catalase (+/-) Staphylococcus aureus | positive Streptococcus pyogens | negative 2) Try the virtual "oxidase test" multiple times to check all following bacteria. Majority of bacteria listed below are human pathogens (We can do easily in the virtual lab.). You probably need to re-start several times to see all bacteria on this list. bacteria | oxidase (+/-) Pseudomonas aeruginosa | positive Neisseria gonorrhoeae | positive Yersinia pestis | negative Salmonella typhi | negative Serratia marcescens | negative Neisseria megingitidis | positive Klebsiella pneumoniae | negative Enterobacter aerogenes | negative Escherichia coli | negative 3) In addition to the production of hydrogen peroxide, what other harmful species of oxygen can be produced by alternative pathways of the electron transport chain, and how are they neutralized? 4) The oxidase test must be read within 20 seconds of starting the experiment. How would you interpret an oxidase test that developed a coloar change at 40 seconds? Explain your answer.
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