RE: Colonies of Neisseria sp. turn purple when a redox dye is applied. The color change is indicative of the activity of the bacterial enzyme: Group of answer choices Beta-galactosidase Urease Cytochrome oxidase Phenylalanine deaminase
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The question involves colonies of Neisseria sp. and their reaction to a redox dye, which indicates the activity of a specific bacterial enzyme. Show more…
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Interpretations of results (color changes): β-galactosidase, produced by N. lactamica, hydrolyzes 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Xgal) to produce a blue-colored end product. N. meningitidis produces γ-glutamylaminopeptidase which hydrolyzes γ-glutamyl-p-nitroanilide (GPNA) to produce a yellow p-nitroaniline end product. Hydroxyprolylaminopeptidase, produced by N. gonorrhoeae, hydrolyzes a β-naphthylamino acid derivative (prolyl-4-methoxynaphthylamide [PMNA]) to release a free β-naphthylamine derivative which then complexes with a diazonium salt (in the red cap) to produce a red-pink color. M. catarrhalis has none of these enzymes; therefore, M. catarrhalis produces no distinct color change in this test.
Adi S.
Question 14: While doing Phenol Red test, next day you observed that the medium of some tubes changed color. Which color change of the following will prove that acid was produced during fermentation? Red to yellow Yellow to red Pink to green Blue to yellow Question 15: You inoculated bacterium in a medium which liquefied next morning due to the presence of an extracellular ____ enzyme. Urease Gelatin ligase Tryptophanase Gelatinase Question 16: What gas will be collected in Durham tube when some cells are grown in carbohydrates? Carbon-dioxide Sulfur-di-oxide Nitric oxide Oxygen
When grown on urea agar, the control organism used on lab #9, turned the medium pink due to the accumulation of ammonia. To detect the presence of oxidase, an enzyme needed for aerobic respiration, an artificial acceptor is used that changes purple if positive. To detect the presence of phenylalanine deaminase, drops of should be added to the tube, resulting in a green precipitate. The control organism for the lipase test was . A halo was observed around it indicating the breakdown of olive oil in the medium. If an organism is positive for the mixed acid test, it is most likely negative for the test. In the nitrate reduction test, the control organism, , is able to reduce nitrates into nitrites. The medium used to detect the presence of proteases was The medium used to detect the presence of amylase was The medium used to detect the presence of lipases was To detect the presence of catalase, drops of should be added to a slide containing the organism in question and the presence of oxygen bubbles should be detected if the enzyme is presence.
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