00:01
We have our template dna strand, and we're going to answer the following questions.
00:04
So i'm trying to find the complementary dna strand.
00:07
If our first strand is running 3 prime to 5 prime, our next strand or our complementary strand of that, we'll start at our 5 prime end.
00:15
And a always pairs with t, adenine always pairs with thymine, and cytosine always pairs with guanine.
00:23
So essentially, just to save some time here, i've went and paired up.
00:26
Again, a always pairs with t or vice versa.
00:29
And c always pairs with g.
00:32
Now, when we do our mrna strand, we're still looking at that template strand, so the one in black.
00:37
But the main difference here is that in mrna, we don't have thymine.
00:44
We instead have uracil.
00:46
So, instead of a pairing to t, a is going to pair to you.
00:52
But again, we're still looking at that black strand up at the top.
00:55
A pairs of t, c pairs with g.
01:01
And then again, our big difference here being any time a is going to pair with you and mrna instead of thymine.
01:11
And then, again, 5 prime end, 3 prime end, they always run anti -parallel to each other.
01:17
Once we have our mrna sequence, we can then determine the amino acid sequence.
01:22
What we have to do is use our fancy little codon table, which is right down here, and also split up our m rna into groups of three called codons...