Use the following genomic DNA sequence to answer the...

Use the following genomic DNA sequence to answer the questions. 1 2 3 4 5 A 5----------CTTGGTATTCAATAGCTTAAGTTCATACGTGC----------3 B 3----------GAACCATAAGTTATCGAATTCAAGTATGCACG----------5 1.) In which direction is RNA transcribed? 2.) Which of the two strands (A or B) serves as the TEMPLATE strand for the transcription of a mRNA that contains both a start and a stop codon? 3.) Which number (1, 2, 3, 4, or 5) best approximates the location of the -10 consensus sequence? 4.) How many amino acids long is the protein encoded by the mRNA from this DNA sequence? 5.) What is the second amino acid encoded by the mRNA from this DNA sequence? 6.) What is the tRNA anticodon sequence that binds to the third codon of the transcribed mRNA? Question 4 What are the two remaining stop codons that were NOT used in the previous question?

Transcript

00:01 So let's answer now the question.

00:02 So in this problem, we have several questions.

00:05 Let's start with the first one.

00:07 So in the first question, we are asked, what is the direction of the rna transcription? so take note that all rna transcription happens in a 5 prime to 3 prime direction.

00:21 So our answer here is 5 prime to 3 prime direction.

00:26 And for the second question, we are asked, which of the two strands will serve as the template strand for making the mrna.

00:39 The strand that will serve for the transcription of the mrna is we have the strand a.

00:48 So strand a.

00:51 So strand a is the transcription or template for transcription.

01:05 Okay, so this is the template for transcription.

01:09 And the strand b is the complementary strand to that mrna.

01:18 So the template strand is complementary to the, sorry, the strand a rather, is complementary to mrna because strand a is the template for the transcription or making of the mrna.

01:32 Well, the strand b is not the template strand.

01:35 And for the third question, okay, the third question, the third.

01:41 Third question is asking us which number best approximates the location of the 10 consensus sequence so for this question the 10 consensus sequence is located upstream to the transcription and the start site is the transcription start site is five so our answer here is we have five and for the fourth question we have here we have here the mrna sequence.

02:13 So let us write first the mrna sequence.

02:17 So we have 5 prime, a -u -g, a -a -c -u -a -a -c -u -a.

02:29 And then we have, let me check again, a -u -g, a -a -c -u -a, and then followed by ag -a -g, a -g -g -g -u -a -c, and then followed by a -g -g -g -c, and then we have u -a -u okay u -a -u and then we have u -g -a -3 -prime and if we are going to translate this into amino acid the amino acid for a -u -g we have met a -a -c is we have asn u -u -a is we have l -e -u -a is we have l -e -u -u -a.

03:17 U -a -g -c, we have s -e -r, and then we have the u -a -u is we have t -i -r, and then we have the stap -c -o -g -a.

03:35 So we have 1, 2, 3, 4, 5.

03:40 So our answer for the fourth question is we have 5 amino acids.

03:45 So our answer here is five amino acids.

03:50 Okay, for the fifth question, okay? for the fifth question, we are asked, what is the second amino acid encoded by the mrna? so the second amino acid here is this amino acid...

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