00:01
Hello everyone, in this question here we have been given two conditions.
00:05
So, a 3d printable hydrogels are shown here for biomedical and bioremediation application.
00:13
So, to test the utility of this system you engineer a strain of p -puteda to utilize the phenol.
00:21
So, here we have been given three panels.
00:23
The first here asked that which condition, the first incubation, second or free cells resulted in the best growth and how did you make that determination and which condition resulted in the best degradation of phenol and how did you make the determination.
00:42
So, basically free bacterial cell shows the best growth.
00:48
The free bacterium shows higher level of phenol degradation.
00:56
The free bacterium here is used as a control and the initial phenol entirely degraded into biomass after 40 hours in the control experiment.
01:08
The od measured in the grid after the first inoculation is 50 percent of the value observed for the liquid free bacteria.
01:22
Thus, we conclude that the free bacteria produce more od and it is due to increase bacterial growth.
01:30
This also suggests that the immobilization of bacteria inside a highly viscous gel reduces the formation of cellulose microfibrils by restricting the cell locomotion here in the culture medium.
01:50
And at the intermediate constituent concentration of 4 .5 wt percentage flink a highly interconnected bacterial cellulose network is formed and thus it maintains a high enough elasticity and yields stress to ensure the printing accuracy.
02:10
So, in this case the ink constituents can be dissolved in water leaving a bacterial cellulose couple.
02:18
So, above 6 wt percent flink the bacterial cellulose formation is not high enough to remain cohesive after submersion of the, submersion in the water...