Sampling lake water for microscopic organisms is not as easy as it sounds when you want to both count and identify species. In a given water sample, your bacteria of choice can be rare or difficult to culture, particularly given the mixture of bacteria in a biofilm. Which of the following culture media would be best for growing fecal coliforms when they are relatively less abundant than other bacteria in the lake? basic culture medium complex culture medium enrichment culture medium anaerobic culture medium
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When they are less abundant than other bacteria in the lake, we need a culture medium that will selectively promote their growth while inhibiting the growth of other bacteria. A basic culture medium is a simple medium that provides the essential nutrients for Show more…
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Adi S.
Objective: Determine the number of bacteria within a 250ml sample of lake water to provide a quantitative risk assessment of fecal coliforms present in a public swimming area. Technical Data: One ml of the lake water sample was passed through a 0.2 micron paper filter disc via vacuum pressure, capturing any bacterial cells on the filter surface. The filter was then placed on an agar plate to support fecal coliform growth for 24 hours at 30 degrees Celcius. After the 24 hour period, 10 coliforms were apparent. From the experimental data, calculate how many bacteria are within the original lake water sample, arriving at a fecal coliform/ml of water. Based on the following water safety guidelines of no more than 200 bacteria/ml, is the lake safe for public swimming? Yes or No.
Jenny W.
1. Briefly explain how you can determine the presence of coliform bacteria in a water sample. 2. What is the purpose of the confirmed test in an experiment designed to test for coliform bacteria? 3. Explain why it is of prime importance to analyze water supplies that serve industrialized communities. 4. When using Endo agar as the selective and differential media for the confirmed test, how would you know whether the test is positive for the presence of E. coli?
Josee P.
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