Select the scenario when a quadrant streak plate would be useful. Identifying contamination in a culture. Performing a Kirby-Bauer experiment. Making a lawn of bacterial growth on a plate. Determining the number of different microbes growing in a patient's wound from a swab sample of the wound AND Identifying contamination in a culture. Determining the number of different microbes growing in a patient's wound from a swab sample of the wound.
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Step 1: A quadrant streak plate is useful for making a lawn of bacterial growth on a plate, as it allows for the isolation and observation of individual bacterial colonies. Show more…
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Madhur L.
After performing the streak plate method of a mixed culture, you obtain an isolated colony. After performing the Gram stain on a sample of the colony, you found that this colony is a mixture of two bacterial types. What is the proper step to take in order to obtain a pure culture from this mixed colony? A) Discard the plate and re-streak using the original mixed culture and the zigzag method using a cotton swab. B) Using your microscope, separate the Gram negative and Gram positive cells with a wire loop. C) It is not necessary to do anything further; you can work with bacteria on the edges where there will be only one type of bacteria and not a mixture. D) Using some of the colony as your inoculum, re-streak onto a fresh plate to separate the two types as two isolated colonies. E) None of the above are proper steps to take.
Shyam P.
If you completed quadrant streak plate showed two different and distinct colon appearance, what could you conclude? Select all that apply A. You initial bacterial culture inoculum contained two unique bacteria species. B. Your technique was poor, as correct quadrant streak plate protocol would have eliminated all but one species in the sample. C. Your Bunsen burner flame was too hot and caused growth of bacteria too early during the streaking. D. Your plate was inoculated with a single species, but break in aseptic technique could have allowed contaminant bacteria to also grow
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