After performing the streak plate method of a mixed culture, you obtain an isolated colony. After performing the Gram stain on a sample of the colony, you found that this colony is a mixture of two bacterial types. What is the proper step to take in order to obtain a pure culture from this mixed colony?
A) Discard the plate and re-streak using the original mixed culture and the zigzag method using a cotton swab.
B) Using your microscope, separate the Gram negative and Gram positive cells with a wire loop.
C) It is not necessary to do anything further; you can work with bacteria on the edges where there will be only one type of bacteria and not a mixture.
D) Using some of the colony as your inoculum, re-streak onto a fresh plate to separate the two types as two isolated colonies.
E) None of the above are proper steps to take.