00:01
Hello students, in this question we have been told that you have coaxed a mammalian tissue, tissue cell culture to take up plasmid that is pgfp.
00:25
So this is green fluorescent protein and we have been told that the plasmid has made its way to the nucleus.
00:36
So now will the gfp that is green fluorescent protein be produced by the mammalian cell? why or why not? so first of all even though the plasmid is taken up by the nucleus, the processing of the unit, the processing of this plasmid to be utilized for the synthesis of protein is very less likely because it is, now the cell considers it as an external, external material.
01:22
So what will it do? it will try to digest this material.
01:26
So now there are proteins that are working for against this plasmid.
01:35
Now this plasmid does not have the ability to hijack host machinery like a virus.
01:48
So this protein will not incorporate itself to the host dna and if it is not a part of the host dna, the mrna for this plasmid will not be formed and because of that there will be no protein as well.
02:06
So because of the complexity of mammalian cell, it is very unlikely for the plasmid to be incorporated into the dna host, dna of the host and then be converted to mrna for the production of protein.
02:27
So this is very unlikely, very rare.
02:31
The chances of this occurring is almost negligible...