Suppose you have a patient who has been poisoned with either DNP or cyanide, but you do not know which. Recall that cyanide inhibits the oxidation of complex IV by oxygen, leading to an accumulation of reduced ETC components. By contrast, DNP disrupts the hydrogen ion gradient in mitochondria without interfering with the activity of the ETC.
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Suppose you have a patient who has been poisoned with either DNP or cyanide, but you do not know which. Recall that cyanide inhibits the oxidation of complex IV by oxygen, leading to the accumulation of reduced ETC components. By contrast, DNP disrupts the hydrogen ion gradient in mitochondria without interfering with the activity of the ETC. Would you expect to see the following cells exposed to DNP, cyanide, both, or neither assuming you could test these conditions in the patient's cells? Drag and drop the correct word next to the corresponding observations. Note: each answer may be used once, more than once, or not at all. - Increased activity of regulated enzymes in glycolysis - Decreased cellular ATP production - Increased production of lactic acid
Marisa A.
Cyanide ion (CN-) blocks electron transfer in mitochondria at the level of Complex IV of the ETC. It essentially prevents the formation of peroxide intermediate during H2O formation. 2,4-Dinitrophenol (DNP) is an uncoupler of mitochondrial oxidative phosphorylation - it allows H+ to diffuse across the inner mitochondrial membrane, but without generating ATP. Draw TWO SEPARATE graphs (ATP synthesized vs. time AND O2 consumed vs. time) with labeled curves to show the effects of adding each of these compounds separately to a suspension of mitochondria supplied with O2, succinate, ADP, and Pi. Use an arrow to indicate the time of drug addition. (10 pts)
Sri K.
We saw that cyanide poisoning affected the capacity of cells to perform oxidative phosphorylation by blocking complex IV of the ETC. 1. What would happen if instead of complex IV, we found that a poison affected complex I? 2. What do you expect would happen to those cells? (Would they live or die, and why?)
Jenny W.
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