2. Complete the following virtual lab: Cell Homeostasis Virtual Lab Fill in the table with the measurement data from the virtual lab Solution Weight of Weight of Net Change in Tonicity of dialysis bag dialysis bag Weight (be sure to Environment before after include is value is + or -) A 17.59g 17.66 0.10g Isotonic B 8.75g 10.40 1.65g Hypotonic C 11.24g 12.10 0.86g Hypotonic D 10.71g 10.57 -0.14g Isotonic E 18.05g 15.60 -2.45 Hypertonic 1. What was the control in the cell homeostasis lab? Pure water 2. Did glucose move across the membrane? No 3. Did water move across the membrane? Yes 4. Why was water able to move across the membrane but glucose could not? 5. How does the rate of diffusion effect the rate of osmosis? 6. This experiment uses a type of tubing called dialysis tubing. How is dialysis tubing used for people that have renal (kidney) failure that have to undergo hemodialysis?
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This means that the dialysis bag was filled with pure water and placed in an environment with the same tonicity as the water inside the bag. Show more…
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Osmosis Data Sheet (Activity 2) Table 2: Dialysis Tube Deionized Water Dialysis Tube 20% Glucose Initial Mass (g) Final Mass (g) Change in Mass (g) Laboratory Questions 1. Explain why you think there was a change in mass for both dialysis tubes. 2. What do you think would happen to the mass of each tube (one with deionized water and the other with 20% glucose) if the two were placed in: Deionized Water 20% Glucose Deionized Water 30% Glucose 5% Glucose 3. A large quantity of sugar is needed to make jam. The cytoplasm of bacteria contains much less sugar. a. Is the bacterial cytoplasm isotonic, hypertonic, or hypotonic to the jam? b. Why might many types of bacteria have difficulty colonizing jams? 4. Suggest ways that we could improve this investigation. How could we be more precise, or make our observations more accurate?
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1a) Dialysis tubing can be used to make bags that model a cell with a semipermeable membrane. These membranes are impermeable to sucrose but not water. Four bags were filled with the following solute concentrations: Bag A – 10 ml of 1% sucrose Bag B – 10 ml of 20% sucrose Bag C – 10 ml of 45% sucrose Bag D – 10 ml of 80% sucrose Each bag was weighed before placing them in a beaker: Bags B and C were placed in a beaker containing 80% sucrose Bags A and D were placed in a beaker containing 1% sucrose At 15 minute intervals, each bag was blotted dry, weighed, and returned to the same beaker. Q.) After 1 hour – did the weight of each bag change from the initial weight and if it did, how did it change, and why? Be sure to include the terms isotonic, hypotonic, and hypertonic.
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