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7. The absorbance of a blue solution at various concentrations is measured using a spectrophotometer set at a particular wavelength. Would a dark blue solution have a higher or lower absorbance reading compared to a less concentrated light blue solution? Defend your answer using your knowledge of how a spectrophotometer works. 8. You are a scientist doing an experiment that requires measuring a variety of volumes. You have at your disposal a 1 L graduated cylinder, a 100 ml graduated cylinder, a 1 ml pipette, a 10-ml pipette, a P-200 micropipette, and a P-20 micropipette. Indicate for each volume the preferred piece of equipment to use from the list above along with a brief explanation. Volume to be measured Equipment to use Explanation 1. 75 ml 2. 500 ml 3. 500 µl 4. 15 µl 5. 5 µl 6. 150 µl 7. 8 ml

          7. The absorbance of a blue solution at various concentrations is measured using a spectrophotometer set at a particular wavelength. Would a dark blue solution have a higher or lower absorbance reading compared to a less concentrated light blue solution? Defend your answer using your knowledge of how a spectrophotometer works.

8. You are a scientist doing an experiment that requires measuring a variety of volumes. You have at your disposal a 1 L graduated cylinder, a 100 ml graduated cylinder, a 1 ml pipette, a 10-ml pipette, a P-200 micropipette, and a P-20 micropipette.

Indicate for each volume the preferred piece of equipment to use from the list above along with a brief explanation.

Volume to be measured	Equipment to use	Explanation
1. 75 ml
2. 500 ml
3. 500 µl
4. 15 µl
5. 5 µl
6. 150 µl
7. 8 ml

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7. The absorbance of a blue solution at various concentrations is measured using a spectrophotometer set at a particular wavelength. Would a dark blue solution have a higher or lower absorbance reading compared to a less concentrated light blue solution? Defend your answer using your knowledge of how a spectrophotometer works.

8. You are a scientist doing an experiment that requires measuring a variety of volumes. You have at your disposal a 1 L graduated cylinder, a 100 ml graduated cylinder, a 1 ml pipette, a 10-ml pipette, a P-200 micropipette, and a P-20 micropipette.

Indicate for each volume the preferred piece of equipment to use from the list above along with a brief explanation.

Volume to be measured Equipment to use Explanation
1. 75 ml
2. 500 ml
3. 500 µl
4. 15 µl
5. 5 µl
6. 150 µl
7. 8 ml

Added by Brian P.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Madhur L

03/03/2023

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For the first part of the question, a dark blue solution would have a higher absorbance reading compared to a less concentrated light blue solution. This is because the spectrophotometer works by measuring the amount of light absorbed by the solution at a specific Show more…

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The absorbance of a blue solution at various concentrations is measured using a spectrophotometer set at a particular wavelength. Would a dark blue solution have a higher or lower absorbance reading compared to a less concentrated light blue solution? Defend your answer using your knowledge of how a spectrophotometer works. You are a scientist doing an experiment that requires measuring a variety of volumes. You have at your disposal a graduated cylinder (100 ml), a pipette (10-ml), a P-200 micropipette, and a P-20 micropipette. Indicate for each volume the preferred piece of equipment to use from the list above, along with a brief explanation. Volume to be measured Equipment to use Explanation 75 ml Graduated cylinder (100 ml) The graduated cylinder is suitable for measuring larger volumes, and it can accurately measure 75 ml. 500 ml Graduated cylinder (100 ml) The graduated cylinder can measure up to 100 ml, so multiple measurements can be made to reach 500 ml. 500 µl Pipette (10-ml) The pipette is suitable for measuring smaller volumes, and it can accurately measure 500 µl. 5.5 µl P-20 micropipette The P-20 micropipette is designed for measuring very small volumes, such as 5.5 µl. 8 ml Graduated cylinder (100 ml) The graduated cylinder can accurately measure 8 ml. 15 µl Pipette (10-ml) The pipette is suitable for measuring smaller volumes, and it can accurately measure 15 µl. 150 µl P-200 micropipette The P-200 micropipette is designed for measuring larger volumes, such as 150 µl.

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