Question

6. The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown. a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments? N E A E 0 2 4 6 8 10 12 kb A E N E 0 2 4 6 8 10 12 kb E A N E 0 2 4 6 8 10 12 kb N A E E 0 2 4 6 8 10 12 kb A N E E 0 2 4 6 8 10 12 kb E A N N A E 0 2 4 6 8 kb Fragment size (kb) 9 8 7 6 5 4 3 2 1 0.5 A N A+N E E+N E=EcoRI N=NotI A=AatII Autoradiograph of Southern blot filter. b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph. Fragment size (kb) 9 8 7 6 5 4 3 2 1 0.5 A N A+N E E+N Where is the pep gene located in the correct restriction map?

          6. The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown.
a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments?
N E A E
0 2 4 6 8 10 12 kb
A E N E
0 2 4 6 8 10 12 kb
E A N E
0 2 4 6 8 10 12 kb
N A E E
0 2 4 6 8 10 12 kb
A N E E
0 2 4 6 8 10 12 kb
E A N N A E
0 2 4 6 8 kb
Fragment size (kb)
9 8 7 6 5 4 3 2 1 0.5
A N A+N E E+N
E=EcoRI N=NotI A=AatII
Autoradiograph of Southern blot filter.
b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph.
Fragment size (kb)
9 8 7 6 5 4 3 2 1 0.5
A N A+N E E+N
Where is the pep gene located in the correct restriction map?

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6. The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown.
a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments?
N E A E
0 2 4 6 8 10 12 kb
A E N E
0 2 4 6 8 10 12 kb
E A N E
0 2 4 6 8 10 12 kb
N A E E
0 2 4 6 8 10 12 kb
A N E E
0 2 4 6 8 10 12 kb
E A N N A E
0 2 4 6 8 kb
Fragment size (kb)
9 8 7 6 5 4 3 2 1 0.5
A N A+N E E+N
E=EcoRI N=NotI A=AatII
Autoradiograph of Southern blot filter.
b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph.
Fragment size (kb)
9 8 7 6 5 4 3 2 1 0.5
A N A+N E E+N
Where is the pep gene located in the correct restriction map?

Added by Colin M.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Tracy Lin

10/09/2023

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Look at the agarose gel and identify the sizes of the restriction fragments. Show more…

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The agarose gel at the right shows the pattern of bands obtained when a particular fragment of double-stranded DNA is digested to completion with the restriction enzymes shown. a. Which of the six restriction maps shown below is consistent with the observed pattern of restriction fragments? b. The DNA from this gel was then blotted onto a nylon filter. The filter was probed at high stringency with a radioactively labeled DNA sequence from the gene pep, which is known to lie somewhere within the region of DNA being studied here. The image to the right shows the pattern of radioactivity observed on the autoradiograph. Where is the pep gene located in the correct restriction map?

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Transcript

00:01 For question number six, it said the agarose gels at the right show the pattern of bands obtained when a particular fragments of double -stranded dna is digested to completion with the reaction enzyme shown here.

00:17 6a asks which of the six reaction maps shown below is consistent with observed pattern of reaction fragments.

00:27 So i'm going to use, check a few.

00:30 So we're going to try the e.

00:31 Coli one first.

00:34 So e.

00:34 Coli one is the one right here, a single digestion, give you three bands here.

00:43 So they are six, five, and one.

00:49 So now i look at here that the e.

00:53 Coli one that in this gel band qualify is going to be this because you have a six fragment, 6kb here, then you have 5kb here, then you have 1kb here.

01:09 And so this is one of the potential candidates.

01:13 So in fact, the first one, the second one, the third one, fourth one, and fifth one, they all qualify for that standard.

01:25 So i have to go on with the second enzyme and i'm going to pick a, which is aat2 enzyme.

01:40 So for a, that you have two bands, one is 9kb and the other one is 3kb.

01:49 So we're going to see which one of the five that will have 9 in 3 fragments.

01:59 So looking at here, the first one, the a is at 8kb point, so that right away eliminate a.

02:10 The second one that you have, the a is at the 3kb.

02:18 So a right here, you have 9 and 3.

02:21 So you have a 3kb cut, then you have a 9 for the rest of the fragments...

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