Question

The Beer-Lambert law is valid for absorbance at any wavelength (as long as the same wavelength is used for all solutions being compared). Thus, any wavelength could be used to generate a linear plot of A vs c. Why was it important in this experiment to first find the absorption maximum and use this wavelength (or one close to the maximum) for generating the Beer-Lambert plot used in these calculations? To help answer this, consider what would happen if you repeated the experiment using absorbance data at 550 nm. A. Any wavelength would work as well. B. The absorption max wavelength gives the largest slope and sensitivity. C. EDMAB only absorbs at the absorption max wavelength.

          The Beer-Lambert law is valid for absorbance at any wavelength (as long as the same wavelength is used for all solutions being compared). Thus, any wavelength could be used to generate a linear plot of A vs c. Why was it important in this experiment to first find the absorption maximum and use this wavelength (or one close to the maximum) for generating the Beer-Lambert plot used in these calculations? To help answer this, consider what would happen if you repeated the experiment using absorbance data at 550 nm.

A. Any wavelength would work as well.

B. The absorption max wavelength gives the largest slope and sensitivity.

C. EDMAB only absorbs at the absorption max wavelength.

The Beer-Lambert law is valid for absorbance at any wavelength (as long as the same wavelength is used for all solutions being compared). Thus, any wavelength could be used to generate a linear plot of A vs c. Why was it important in this experiment to first find the absorption maximum and use this wavelength (or one close to the maximum) for generating the Beer-Lambert plot used in these calculations? To help answer this, consider what would happen if you repeated the experiment using absorbance data at 550 nm.

A. Any wavelength would work as well.

B. The absorption max wavelength gives the largest slope and sensitivity.

C. EDMAB only absorbs at the absorption max wavelength.

Added by Rebecca G.

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