00:01
Everyone in this question we will be talking about the experiments of the john keynes that confirmed the dna replication.
00:11
So keynes basically used the autoradiography to show the vaccinia virus dna replication in factories that is located in the cytoplasm and he proved that the bacterial chromosome comprises of a single growing point and the bidirectionality of the chromosome replication.
00:34
So here our right answer would be option a, bidirectionality because in the bacteria the replication takes place in a bidirectional manner.
00:47
Then the next question says the experiments by robert okazaki confirmed that dna replication.
00:55
He used the pulse shape experiment to confirm about the discontinuous strand of the dna which is synthesized on the template which is in 5 prime to 3 prime direction.
01:09
So here this is known as the lagging strand of the discontinuous strand.
01:15
So here our right answer would be option b that is this continuously.
01:22
Then in the next question it says that during replication in e .coli the initial separation of the double helical dna as the origin is accomplished by the action of.
01:38
So here basically the dna is present in double stranded chains like this.
01:43
So there needs the enzyme which will basically break the hydrogen bond and this here is done by the activity of the enzyme which is known as henry case.
01:55
So our right answer is option a that is henry case.
02:00
Then in the next question it says during replication in the e .coli the construction of short rna chain is accomplished by.
02:09
So basically this is oligonucleotide which is required for the division of the 3 prime oh in for dna polymerase to add the nucleotide.
02:20
So it is the primase which adds the oligonucleotide which is known as the primer.
02:28
So our right answer is that is primase.
02:33
So this primase adds the primer here.
02:35
Then in the next question it says the molecules constructed in question 14 above are that are these oligonucleotide which are basically the rna molecules and are known as primers.
02:48
So our right answer is option c that is primers.
02:52
Then in the next question it says that we have 30 % of the guanine monophosphate.
03:04
So according to the chagas rule the guanine will be equal to the amount of the cytosine and adenine will be equal to the amount of the thymine because guanine forms triple bond with cytosine and adenine forms double hydrogen bond with thymine.
03:23
So if it is 30 % guanine that means 30 % dcmp must be present and that means here we are left with rest 40%.
03:34
So that will be divided into 20 % adenine and 20 % thymine.
03:40
So here we have been asked about the dtmp.
03:44
So that will be option c that is 20%...