The figure 1 below is referred to a double-stranded DNA contains sequence of a eukaryotic gene: b 1 2 3 5'-ATGGCCTTCACACAGGAAACAGCTATGGCCATGAGCACGC 1 ----+----+----+----+ 40 3'-TACCGGAAGTGTGTCCTTTGTCGATACCGGTACTCGTGCG ii CAGTCTCGGCATTATCCTATTAAAGGGAACTGAGGTGA-3' 41 ----+----+----+----+ 80 GTCAGAGCCGTAATAGGATAATTTCCCTTGACTCCACT-5' i Figure 1: Double-stranded DNA a. In the figure 1, the transcription begins at the underlined A/T at base pair 17 (b) and proceeds to the right. Derive the resulted mRNA with indication of 5' and 3' ends. (2 marks) b. Encode the amino acids from the generated mRNA (a) with complete label of amine terminus and carboxyl terminus based on the amino acid table provided (Figure 2). (4 marks) c. Briefly explain how would the resulting protein change if the underlined G/C base pair at position 22 (1) is deleted from the DNA sequence. (2 marks) d. Briefly explain how would the resulting protein change if the underlined G/C base pair at position 27 (2) is changed to a C/G base pair in the DNA sequence. (2 marks)
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First, we need to find the template strand of DNA that will be transcribed into mRNA. Since transcription begins at the underlined AT at base pair 17 and proceeds to the right, the template strand is the bottom strand (3' to 5' direction). Show more…
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