Question

The following observations are made on an unknown membrane protein, Protein AB. It was found that protein AB could extracted from disrupted erythrocyte membranes and cleaved into fragments by proteolytic enzymes. Two experiments were conducted. Experiment 1: Treatment of erythrocytes with proteolytic enzymes followed by disruption and extraction of membrane components yielded almost all intact AB. Experiment 2: Treatment of ISOLATED erythrocyte plasma membranes (produced by disrupting the cells and washing out the hemoglobin) with proteolytic enzymes, followed by disruption and extraction, yielded fragmented AB. What do these observations indicate about the location of Protein AB in the plasma membrane? Do the structural properties resemble those of an integral or peripheral membrane protein?

          The following observations are made on an unknown membrane protein, Protein AB. 
It was found that protein AB could extracted from disrupted erythrocyte membranes
and cleaved into fragments by proteolytic enzymes. Two experiments were 
conducted. 
Experiment 1: Treatment of erythrocytes with proteolytic enzymes followed 
by disruption and extraction of membrane components yielded almost all
intact AB.
Experiment 2: Treatment of ISOLATED erythrocyte plasma membranes
(produced by disrupting the cells and washing out the hemoglobin) with 
proteolytic enzymes, followed by disruption and extraction, yielded
fragmented AB. 
What do these observations indicate about the location of Protein AB in the plasma 
membrane? Do the structural properties resemble those of an integral or peripheral 
membrane protein?

Added by Victoria C.

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