Question

The linear concentration range for the Bradford assay's standard curve is usually 0.1-1.0 mg/mL of protein. Concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains about 5 mg/mL of protein, how can you dilute this sample to 0.5 mg/mL? Show your answer using a single dilution using water. The final volume of the dilution should be 1 mL. Answers are to be shown to one decimal place only. For example: If you think the volumes of protein and water required are 0.3 mL and 0.7 mL, respectively, then just enter 0.3 and 0.7 in the appropriate blank spaces.

          The linear concentration range for the Bradford assay's standard curve is usually 0.1-1.0 mg/mL of protein. Concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains about 5 mg/mL of protein, how can you dilute this sample to 0.5 mg/mL? Show your answer using a single dilution using water. The final volume of the dilution should be 1 mL. Answers are to be shown to one decimal place only. For example: If you think the volumes of protein and water required are 0.3 mL and 0.7 mL, respectively, then just enter 0.3 and 0.7 in the appropriate blank spaces.

Added by Francisco Jose B.

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