The production of ROS can be reduced using drugs that allow protons to freely pass through the inner membrane. The effect of a drug called DNP on the life span of mice is shown in the graph below. Each point represents the death of a single mouse. At what age is the difference in survival between the DNP-treated and the untreated control the greatest?
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Adi S.
How could you determine if the mitochondrial ETC is affected in DNP-treated mice? Propose an experiment to determine if there is a correlation between life span and ETC activity in mitochondria isolated from mice used in the experiment above.
The graph below shows data on the effect of DNP on mitochondrial function in human myotubule cells (muscle cell progenitors). For this experiment, human myotubules were grown in the presence ("DNP treated") or absence ("Control") of DNP. The levels of ATP and glucose oxidation were measured. The data are expressed as a percent of the control level. The error bars show the standard error (n= 3 for ATP analysis, n=8 for glucose oxidation). ATP Glucose Oxidation Control DNP Treated Summarize what happens to ATP and glucose oxidation levels in response to treatment with DNP. How does DNP cause the effects shown in the graph? Think mechanistically!
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