The scientist then loads a gel with a pH gradient and separates the same set of proteins with isoelectric focusing. Arrange the proteins by expected order of migration from the cathode to the anode. Cathode (-) Anode (+) Answer Bank cytochrome c aldolase lactate dehydrogenase phosphoenolpyruvate carboxylase pyruvate kinase ornithine decarboxylase
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The pI is the pH at which the protein has no net charge. Proteins with a lower pI will have a net negative charge and migrate towards the anode (+), while proteins with a higher pI will have a net positive charge and migrate towards the cathode (~). Here are the Show more…
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