The scientist then loads a gel with a ph gradient and...

Name: the scientist then loads a gel with a ph gradient and separates the same set of proteins with isolectric focusing trange the proteins by expected order of migration from ticathode to the ano 60887
Uploaded: 2023-08-21T03:32:59-08:00
Duration: 1 min 8 s
Channel: Madhur L
Description: the scientist then loads a gel with a ph gradient and separates the same set of proteins with isolectric focusing trange the proteins by expected order of migration from ticathode to the ano 60887

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Exhibit 5A The following diagram shows the anode, cathode, and pH gradient of an isoelectric focusing bed: anode+ pH 1 2 3 4 5 6 7 8 9 10 11 12 13 14 -cathode 6. Refer to Exhibit 5A. A mixture of asp, asn, and arg is placed in the bed where the pH is 7, and the current is turned on. From left to right, which best represents the final positions of the individual amino acids? a. asp asn arg b. arg asn asp c. asn asp arg d. arg asp asn 7. In all forms of chromatography one way of identifying eluted substances is by a. fluorescence spectroscopy b. comparison with standards c. radioactive labeling d. treating fractions with a reagent that will cause a color change 8. Differential centrifugation at low speeds (several thousand RPM) is a useful step when a. organelles have been lysed. b. enzymes of interest have different sizes. c. cell membranes must be left intact. d. ribosomes need to be broken down. e. there are either organelles or debris to separate. 9. In isoelectric focusing gel electrophoresis a. particular care must be taken to ensure the same pH along the length of the gel b. there is a pH gradient that parallels the electric field gradient c. the electric current is allowed to fluctuate d. the electric circuits of the apparatus must be very well insulated 10. Ammonium sulfate is useful in protein purification because a. it contains nitrogen and sulfur, both of which occur in proteins b. it is sparingly soluble in water, causing proteins to co-precipitate with it c. very pure proteins are obtained when it is used d. it forms ion-dipole interactions with water, making proteins less soluble and more likely to precipitate 11. The purity of an enzyme at various stages of purification is best measured by a. total protein. b. total enzyme activity. c. specific activity of the enzyme. d. percent recovery of the protein. e. percent recovery of the enzyme.

Madhur L.

the-scientist-then-loads-a-gel-with-a-ph-gradient-and-separates-the-same-set-of-proteins-with-isolectric-focusing-arrange-the-proteins-by-expected-order-of-migration-from-the-cathode-to-the-14645

The scientist then loads a gel with a pH gradient and separates the same set of proteins with isoelectric focusing. Arrange the proteins by expected order of migration from the cathode to the anode. Cathode (-) Anode (+) Answer Bank cytochrome c aldolase lactate dehydrogenase phosphoenolpyruvate carboxylase pyruvate kinase ornithine decarboxylase

Adi S.

9) In size exclusion chromatography, smaller proteins are eluted faster than larger molecules cross-linking of polymer which comprises the stationary phase determines the pore size the most positively charge proteins are eluted last large proteins migrate the slowest through the column 10) In isoelectric focusing, charge on the protein does not change as the protein migrates through the gel a protein has a net positive charge when it reaches a pH equal to its pI proteins can be separated based on their pI a thermal gradient is created in the presence of an electric field

Adi S.

Transcript

00:01 Hello students, in this session it is talking about a mixture of proteins aspartic acid, asparagine and arginine at ph 7 and asking for their order of amino acids after separation.

00:16 It will be aspartic acid, asparagine and arginine.

00:20 Chromatography used to identify eluted substances by comparison with the standards.

00:26 Different centrifugation at low speed takes place because organelle debris needs to be separated...

Question

The scientist then loads a gel with a pH gradient and separates the same set of proteins with isolectric focusing. trange the proteins by expected order of migration from ticathode to the anode.

Question

The scientist then loads a gel with a pH gradient and separates the same set of proteins with isolectric focusing. trange the proteins by expected order of migration from ticathode to the anode.

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