The starch agar protocol is a biochemical test to determine if a microbe secretes \alpha - amylase and
Added by Rosario E.
Step 1
** Show more…
Show all steps
Your feedback will help us improve your experience
Sri K and 60 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
LAB 4.6 QUESTIONS (Out of 3 points) 1. What is the advantage of producing extracellular amylase and glycosidase? 2. Can you substitute soluble starch with glucose in this test? Why or why not? 3. Why is it recommended to mark the growth pattern on the agar plate before pouring iodine?
Sri K.
Describe the function of amylase in starch hydrolysis. What does starch hydrolysis by bacteria indicate? What is the chemical used to detect microbial starch hydrolysis? What are the ingredients of starch agar? How is it possible for bacteria to grow heavily on starch agar but not necessarily produce -amylase?
Adi S.
6.2 - Amylase Activity 5. Review the section in your lab protocol that describes amylase. Based on what you read, predict the results of the amylase plate test. For each of the following, predict whether it will break down the starch in the plate: Saliva: Break down Soaked (germinating) seed: break down Dry (dormant) seed: won't break down Boiled seed: won't break down In this test, is saliva serving as a positive control, or a negative control? Explain. 6. Using the image here, diagram the color changes that occurred after adding iodine, and label what was in each quadrant. Use color (brown or black) to show the location of the starch and the areas where starch was absent. Explain your results thoroughly. 7. Both starch and cellulose are polysaccharides consisting of long chains of glucose, and yet amylase is an enzyme that breaks down only starch. Why can it not break down cellulose? Do a little bit of online research to find out the structural differences between starch and cellulose, address that in your answer, and relate that to enzyme function.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD
