A.) How many recombination events are required for plasmid F to become an F' carrying a single segment of the bacterial chromosome?
My answer: 2
B.) Three genes are arranged in the order abc, mno, and xyz. In a transformation experiment, the DNA is made from a prototrophic strain and used to transform a triple mutant.
C.) How many crossovers would be required to generate a stable strain that is phenotypically abc+ mno- xyz+? In a second transformation experiment, the DNA is made from a triple mutant strain and used to transform a prototrophic strain.
D.) How many crossovers would be required to generate a stable strain that is phenotypically abc+ mno- xyz+? Also, illustrate your answer using a simple diagram to show the crossovers involved.