THE WHOLE QUESTION IS STRUCTURED LIKE THIS: A.) How many recombination events are required for plasmid F to become an F’ carrying a single segment of the bacterial chromosome? My answer: 2 B.) Three genes are arranged in the order abc, mno and xyz. In a transformation experiment the DNA is made from a prototrophic strain and used to transform a triple mutant. C.) How many crossovers would be required to generate a stable strain that is phenotypically abc+ mno- xyz+. In a second transformation experiment, the DNA is made from a triple mutant strain and used to transform a prototrophic strain. D.) How many crossovers would be required to generate a stable strain that is phenotypically abc+ mno- xyz+? also illustrate your answer using a simple diagram to show the crossovers involved.
Added by Jerry M.
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Step 1: In order for plasmid F to become an F' carrying a single segment of the bacterial chromosome, two recombination events are required. Show more…
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A.) How many recombination events are required for plasmid F to become an F' carrying a single segment of the bacterial chromosome? My answer: 2 B.) Three genes are arranged in the order abc, mno, and xyz. In a transformation experiment, the DNA is made from a prototrophic strain and used to transform a triple mutant. C.) How many crossovers would be required to generate a stable strain that is phenotypically abc+ mno- xyz+? In a second transformation experiment, the DNA is made from a triple mutant strain and used to transform a prototrophic strain. D.) How many crossovers would be required to generate a stable strain that is phenotypically abc+ mno- xyz+? Also, illustrate your answer using a simple diagram to show the crossovers involved.
Supreeta N.
Three genes are arranged in the order abc, mno, and xyz. In a transformation experiment, the DNA is made from a prototrophic strain and used to transform a triple mutant. How many crossovers would be required to generate a stable strain that is phenotypically abc+ mno- xyz+? Also, illustrate the answer using a simple diagram to show the crossovers involved.
Adi S.
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