Two phospho-CREBs plus a CREB binding protein bind the CRE. True False
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Two phosphorylated CREB proteins bind to the CREB binding protein, which then binds to the CRE (cAMP response element) in the DNA. Show more…
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Madhur L.
These are results from a CAT assay. Three different transcription factors were tested on one promoter. Three different proteins were used: control, CREB, and DCREB. The control protein had no transcriptional activating activity. CREB is suspected of activating transcription, and a mutant (D) CREB with 14 amino acids deleted from the middle of the protein. Minus (-) indicates no added protein kinase A, while (+) indicates protein kinase A was added to the mixture. Each experiment was performed in duplicate. Interpret these data.
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