Two proteins bind to the same ligand but with different binding affinities
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1. An allosteric interaction between a ligand and a protein is one in which: a. Binding of a molecule to a binding site affects binding of additional molecules to the same site. b. Binding of a molecule to a binding site affects binding properties of another site on the protein. c. Binding of the ligand to the protein is covalent. d. Multiple molecules of the same ligand can bind to the same binding site. e. Two different ligands can bind to the same binding site. 2. Two proteins bind to the same ligand, and protein A has half of its binding sites occupied when the ligand concentration is 0.5 mM, while protein B has half of its binding sites occupied when the ligand concentration is at 0.3 mM. Which protein binds the ligand more strongly, and what is the dissociation constant for that protein-ligand interaction? a. Protein A binds more strongly, and Kd = 0.3 mM. b. Protein A binds more strongly, and Kd = 0.5 mM. c. Protein B binds more strongly, and Kd = 0.3 mM. d. Protein B binds more strongly, and Kd = 0.5 mM. e. Both proteins bind the ligand with the same affinity, and Kd = 0.4 mM.
John N.
Which of the following situations would produce a Hill plot with $n_{\mathrm{H}}<1.0 ?$ Explain your reasoning in each case. (a) The protein has multiple subunits, each with a single ligand-binding site. Binding of ligand to one site decreases the binding affinity of other sites for the ligand. (b) The protein is a single polypeptide with two ligand-binding sites, each having a different affinity for the ligand. different affinity for the ligand. (c) The protein is a single polypeptide with a single ligand-binding site. As purified, the protein preparation is heterogeneous, containing some protein molecules that are partially denatured and thus have a lower binding affinity for the ligand.
The differences between protein A and B in the figure below are best described as: Protein B binds more ligand than A at higher concentrations of L. The Kd for A is larger than the Kd for B with respect to L. The Kd for A binding is approximately 2 uM. Protein A is about twice as fast as protein B at 2 uM. B has higher affinity for ligand L than A.
Adi S.
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