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Questions: 1. What purpose does the Tryptic Soy Agar (TSA) plate serve? observing colony morphology, developing the pure culture and achieving sufficient growth of bacteria. Provides structure for microbial growth. a. How does the TSA plate increase the validity of the test results? 2. Why is the steak plate method preferred over the zig-zag method of plate inoculation for this exercise? 3. With the diversity of microorganisms in the world, how can a single test, such as MSA, be used to confidently identify Staphylococcus aureus? 4. Phenylethyl Alcohol agar (PEA) contains only 0.25% phenylethyl alcohol because high concentrations inhibit both Gram-negative organisms and Gram-positive organisms. List some possible reasons why this is true. 5. For each media inoculated, write a paragraph to discuss the observations. The paragraph should include which organisms were inoculated onto the media, what results were observed, if the results were expected, and what the results reveal about the characteristics of the organism. 6. If any of the results were not expected, write a paragraph to trouble-shoot the problem. The paragraph should state the problem, how the observations were affected, and how the problem can be avoided in the future.

          Questions: 1. What purpose does the Tryptic Soy Agar (TSA) plate serve? observing colony morphology, developing the pure culture and achieving sufficient growth of bacteria. Provides structure for microbial growth. a. How does the TSA plate increase the validity of the test results? 2. Why is the steak plate method preferred over the zig-zag method of plate inoculation for this exercise? 3. With the diversity of microorganisms in the world, how can a single test, such as MSA, be used to confidently identify Staphylococcus aureus? 4. Phenylethyl Alcohol agar (PEA) contains only 0.25% phenylethyl alcohol because high concentrations inhibit both Gram-negative organisms and Gram-positive organisms. List some possible reasons why this is true. 5. For each media inoculated, write a paragraph to discuss the observations. The paragraph should include which organisms were inoculated onto the media, what results were observed, if the results were expected, and what the results reveal about the characteristics of the organism. 6. If any of the results were not expected, write a paragraph to trouble-shoot the problem. The paragraph should state the problem, how the observations were affected, and how the problem can be avoided in the future.

Questions: 1. What purpose does the Tryptic Soy Agar (TSA) plate serve? observing colony morphology, developing the pure culture and achieving sufficient growth of bacteria. Provides structure for microbial growth. a. How does the TSA plate increase the validity of the test results? 2. Why is the steak plate method preferred over the zig-zag method of plate inoculation for this exercise? 3. With the diversity of microorganisms in the world, how can a single test, such as MSA, be used to confidently identify Staphylococcus aureus? 4. Phenylethyl Alcohol agar (PEA) contains only 0.25% phenylethyl alcohol because high concentrations inhibit both Gram-negative organisms and Gram-positive organisms. List some possible reasons why this is true. 5. For each media inoculated, write a paragraph to discuss the observations. The paragraph should include which organisms were inoculated onto the media, what results were observed, if the results were expected, and what the results reveal about the characteristics of the organism. 6. If any of the results were not expected, write a paragraph to trouble-shoot the problem. The paragraph should state the problem, how the observations were affected, and how the problem can be avoided in the future.

Added by Shawn Z.

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