§11a. Right- and left-handed superhelical DNA molecules are known to be negatively and positively supercoiled, respectively. The supercoiled DNA plasmid shown below has 500 base pairs. Calculate the linking number of this plasmid. Is the DNA double helix underwound or overwound? 2 pts.
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The image shows a left-handed supercoil. According to the problem statement, left-handed superhelical DNA molecules are positively supercoiled. Show more…
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Sana R.
For a relaxed 420-base pair DNA circle with an average 10.5 base pairs per helical turn, what is the linking number? For a relaxed 420-base pair DNA circle with an average 10.5 base pairs per helical turn, if the DNA were cut, then one end was twisted by five turns in the direction opposite to the natural helicity of the DNA, and subsequently resealed, what is the linking number?
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a. A closed circular DNA molecule in its relaxed form has a linking number (Lk) of 220. About how many base pairs are in this piece of DNA? (assuming 10.5 bp/turn) b. If the DNA were cut, then one end was twisted by ten turns in the direction opposite to the natural helicity of the DNA, and subsequently resealed. What is the linking number? c. If the relaxed DNA molecule is turned left-handed along the axis of the duplex in the space 10 times, please calculate the linking number. d. How would the linking number (Lk), twist (Tw), and writhe (Wr) of the DNA change when DNA gyrase and ATP are added to the solution? (Possible answers include: increase, decrease, remain the same, and undefined) and why?
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